non tumorigenic tubular epithelial cell line fhs74 (ATCC)
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Non Tumorigenic Tubular Epithelial Cell Line Fhs74, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 78 article reviews
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Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
Journal: medRxiv
doi: 10.1101/2021.03.25.21254204
Figure Legend Snippet: (a) CPLX1 expression levels of mRNA on 14 gastric cancer (GC) cell lines and FHs74, nontumorigenic tubular epithelial cell line. (b) CPLX1-knockdown effect on three GC cell lines at mRNA levels. (c) Proliferation assay comparing those four GC cell lines between untreated, transfected by siControl and siCPLX1. Apoptotic cell observation (d) at cultured on dishes through a phase-contrast microscope, and (e) by staining with annexin-V. Those four GC cell lines were compared between untreated, treated by siControl, treated by siCPLX1. Cells treated by UV-irradiation were positive controls. (f) Migration assay and (g) invasion assay on AGS cells. *P < 0.05, **P < 0.01, ***P < 0.001.
Techniques Used: Expressing, Knockdown, Proliferation Assay, Transfection, Cell Culture, Microscopy, Staining, Irradiation, Migration, Invasion Assay
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Expressing:Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the Knockdown:Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the Proliferation Assay:Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the Transfection:Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the Cell Culture:Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the Microscopy:Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the Staining:Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the Irradiation:Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the Migration:Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the Invasion Assay:Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the |