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non tumorigenic tubular epithelial cell line fhs74  (ATCC)


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    Structured Review

    ATCC non tumorigenic tubular epithelial cell line fhs74
    (a) CPLX1 expression levels of mRNA on 14 gastric cancer (GC) cell lines and <t>FHs74,</t> nontumorigenic tubular epithelial cell line. (b) CPLX1-knockdown effect on three GC cell lines at mRNA levels. (c) Proliferation assay comparing those four GC cell lines between untreated, transfected by siControl and siCPLX1. Apoptotic cell observation (d) at cultured on dishes through a phase-contrast microscope, and (e) by staining with annexin-V. Those four GC cell lines were compared between untreated, treated by siControl, treated by siCPLX1. Cells treated by UV-irradiation were positive controls. (f) Migration assay and (g) invasion assay on AGS cells. *P < 0.05, **P < 0.01, ***P < 0.001.
    Non Tumorigenic Tubular Epithelial Cell Line Fhs74, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+tumorigenic+tubular+epithelial+cell+line+fhs74/BCL2+Jurkat/med_rxiv__2021__03__25__21254204-31-37-48
    Average 99 stars, based on 78 article reviews
    non tumorigenic tubular epithelial cell line fhs74 - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy"

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy

    Journal: medRxiv

    doi: 10.1101/2021.03.25.21254204

    (a) CPLX1 expression levels of mRNA on 14 gastric cancer (GC) cell lines and FHs74, nontumorigenic tubular epithelial cell line. (b) CPLX1-knockdown effect on three GC cell lines at mRNA levels. (c) Proliferation assay comparing those four GC cell lines between untreated, transfected by siControl and siCPLX1. Apoptotic cell observation (d) at cultured on dishes through a phase-contrast microscope, and (e) by staining with annexin-V. Those four GC cell lines were compared between untreated, treated by siControl, treated by siCPLX1. Cells treated by UV-irradiation were positive controls. (f) Migration assay and (g) invasion assay on AGS cells. *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: (a) CPLX1 expression levels of mRNA on 14 gastric cancer (GC) cell lines and FHs74, nontumorigenic tubular epithelial cell line. (b) CPLX1-knockdown effect on three GC cell lines at mRNA levels. (c) Proliferation assay comparing those four GC cell lines between untreated, transfected by siControl and siCPLX1. Apoptotic cell observation (d) at cultured on dishes through a phase-contrast microscope, and (e) by staining with annexin-V. Those four GC cell lines were compared between untreated, treated by siControl, treated by siCPLX1. Cells treated by UV-irradiation were positive controls. (f) Migration assay and (g) invasion assay on AGS cells. *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Expressing, Knockdown, Proliferation Assay, Transfection, Cell Culture, Microscopy, Staining, Irradiation, Migration, Invasion Assay

    Related Articles

    Expressing:

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
    Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.. All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank on June 30th, 2015.All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank..

    Knockdown:

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
    Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.. All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank on June 30th, 2015.All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank..

    Proliferation Assay:

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
    Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.. All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank on June 30th, 2015.All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank..

    Transfection:

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
    Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.. All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank on June 30th, 2015.All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank..

    Cell Culture:

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
    Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.. All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank on June 30th, 2015.All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank..

    Microscopy:

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
    Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.. All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank on June 30th, 2015.All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank..

    Staining:

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
    Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.. All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank on June 30th, 2015.All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank..

    Irradiation:

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
    Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.. All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank on June 30th, 2015.All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank..

    Migration:

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
    Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.. All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank on June 30th, 2015.All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank..

    Invasion Assay:

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy
    Article Snippet: Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.Particularly, the data were retrieved from the GSE62254, GSE15459, GSE22377, GSE62254, GSE29272, GSE38749 limited to UICC stage II-III.. Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.. All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank on June 30th, 2015.All cell lines were tested for mycoplasma, analyzed using the short tandem-repeat-polymerase chain reaction (PCR) method, and authenticated by the JCRB Cell Bank..



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    ATCC non tumorigenic tubular epithelial cell line fhs74
    (a) CPLX1 expression levels of mRNA on 14 gastric cancer (GC) cell lines and <t>FHs74,</t> nontumorigenic tubular epithelial cell line. (b) CPLX1-knockdown effect on three GC cell lines at mRNA levels. (c) Proliferation assay comparing those four GC cell lines between untreated, transfected by siControl and siCPLX1. Apoptotic cell observation (d) at cultured on dishes through a phase-contrast microscope, and (e) by staining with annexin-V. Those four GC cell lines were compared between untreated, treated by siControl, treated by siCPLX1. Cells treated by UV-irradiation were positive controls. (f) Migration assay and (g) invasion assay on AGS cells. *P < 0.05, **P < 0.01, ***P < 0.001.
    Non Tumorigenic Tubular Epithelial Cell Line Fhs74, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+tumorigenic+tubular+epithelial+cell+line+fhs74/BCL2+Jurkat/med_rxiv__2021__03__25__21254204-31-37-48
    Average 99 stars, based on 1 article reviews
    non tumorigenic tubular epithelial cell line fhs74 - by Bioz Stars, 2026-10
    99/100 stars
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    Image Search Results


    (a) CPLX1 expression levels of mRNA on 14 gastric cancer (GC) cell lines and FHs74, nontumorigenic tubular epithelial cell line. (b) CPLX1-knockdown effect on three GC cell lines at mRNA levels. (c) Proliferation assay comparing those four GC cell lines between untreated, transfected by siControl and siCPLX1. Apoptotic cell observation (d) at cultured on dishes through a phase-contrast microscope, and (e) by staining with annexin-V. Those four GC cell lines were compared between untreated, treated by siControl, treated by siCPLX1. Cells treated by UV-irradiation were positive controls. (f) Migration assay and (g) invasion assay on AGS cells. *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: medRxiv

    Article Title: Transcriptomic Profiling on Localized Gastric Cancer Identified CPLX1 as a Gene Promoting Malignant Phenotype of Gastric Cancer and a Predictor of Recurrence after Surgery and Subsequent Chemotherapy

    doi: 10.1101/2021.03.25.21254204

    Figure Lengend Snippet: (a) CPLX1 expression levels of mRNA on 14 gastric cancer (GC) cell lines and FHs74, nontumorigenic tubular epithelial cell line. (b) CPLX1-knockdown effect on three GC cell lines at mRNA levels. (c) Proliferation assay comparing those four GC cell lines between untreated, transfected by siControl and siCPLX1. Apoptotic cell observation (d) at cultured on dishes through a phase-contrast microscope, and (e) by staining with annexin-V. Those four GC cell lines were compared between untreated, treated by siControl, treated by siCPLX1. Cells treated by UV-irradiation were positive controls. (f) Migration assay and (g) invasion assay on AGS cells. *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Gastric cancer cell lines (AGS, RRID is CVCL_0139; GCIY, CVCL_1228; IM95, CVCL_2961; KATOIII, CVCL_0371; MKN1, CVCL_1415; MKN7, CVCL_1417; MKN45, CVCL_0434; MKN74, CVCL_2791; N87, CVCL_1603; NUGC2, CVCL_1611; NUGC3, CVCL_1612; NUGC4, CVCL_3082; OCUM1, CVCL_3084; and SC-6-JCK, CVCL_F953) and the non-tumorigenic tubular epithelial cell line FHs74 (CVCL_2899) were obtained from the American Type Culture Collection (Manassas, VA, USA), the Japanese Collection of Research Bio Resources Cell Bank (Osaka, Japan), or were established at our institute.

    Techniques: Expressing, Knockdown, Proliferation Assay, Transfection, Cell Culture, Microscopy, Staining, Irradiation, Migration, Invasion Assay